How to set null to a GUID property

Choose your poison - if you can't change the type of the property to be nullable then you're going to have to use a "magic" value to represent NULL. Guid.Empty seems as good as any unless you have some specific reason for not wanting to use it. A second choice would be Guid.Parse("ffffffff-ffff-ffff-ffff-ffffffffffff") but that's a lot uglier IMHO.

Guid? myGuidVar = (Guid?)null;

It could be. Unnecessary casting not required.

Guid? myGuidVar = null;

Since "Guid" is not nullable, use "Guid.Empty" as default value.

Is there a way to set my property as null or string.empty in order to restablish the field in the database as null.

No. Because it's non-nullable. If you want it to be nullable, you have to use Nullable<Guid> - if you didn't, there'd be no point in having Nullable<T> to start with. You've got a fundamental issue here - which you actually know, given your first paragraph. You've said, "I know if I want to achieve A, I must do B - but I want to achieve A without doing B." That's impossible by definition.

The closest you can get is to use one specific GUID to stand in for a null value - Guid.Empty (also available as default(Guid) where appropriate, e.g. for the default value of an optional parameter) being the obvious candidate, but one you've rejected for unspecified reasons.