Python print random line from file without repeat

from random import sample

file_name = "text_database.txt"
lines = open(file_name, "r").read().splitlines()

for line in sample(lines, k=len(lines)):
    print(line)

I use .read().splitlines() instead of .readlines() to purge the trailing whitespace (newlines) from each line. I could have also done:

lines = [line.rstrip("\n") for line in open(file_name, "r")]

Here is a description of random.sample from the documentation:

Return a k length list of unique elements chosen from the population sequence. Used for random sampling without replacement.

Alternatively, you could have shuffled your list of lines, and then iterated over them.

EDIT - I think I understand now. How's this?

def main():

    from random import shuffle

    file_name = "text_database.txt"
    lines = open(file_name, "r").read().splitlines()
    shuffle(lines)

    sentinel = object()

    def command_random():
        try:
            line = lines.pop()
        except IndexError:
            print("There are no more lines in the file!")
        else:
            print(line)

    def command_quit():
        nonlocal sentinel
        sentinel = None

    commands = {
        "random": command_random,
        "quit": command_quit
    }

    while sentinel is not None:
        user_input = input("Please enter a command: ")
        command = commands.get(user_input)
        if command is None:
            continue
        command()

    return 0


if __name__ == "__main__":
    import sys
    sys.exit(main())

this is a REALLY messy solution but i have tested this beforehand

f = open(text_database, "r")

list = []
list_of_nums = []

for i in f:
    list.append(i)

elif command == '/random':

    randomNum = random.randint(0, len(list) - 1)

    def reRun():
        global randomNum
        for i in list_of_nums:

            if randomNum == i:
                randomNum = random.randint(0, len(list) - 1)
                reRun()


    reRun()
    list_of_nums.append(randomNum)

    print(list[randomNum])

What this code deos is go throgh all the lines in f and put them in a list. than it choses a random number bettween 0 and the lenght of the list and prints a random line corresponding to that number

Hope this helps! And welcome to stack overflow

elif command == '/random':
    with open (text_database) as f:
        lines = f.readlines()

    while len(lines)>0:
        max_int = len(lines)-1 #update the len each loop as we remove one each time
        print(lines.pop(random.randint(0, max_int))) #pop a random value from the list