I'm getting the error "Application" is ambiguous in the namespace 'microsoft.office.interop.excel'

I don't think you should have the line as Imports Microsoft.Office.Interop. Either use

Imports Excel = Microsoft.Office.Interop.Excel

And then use it as:

Dim xl As Excel.Application

Or remove the Imports all together and use the full name everywhere, as:

Dim xl As Microsoft.Office.Interop.Excel.Application

Removing and re-adding the reference solved this issue.

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