How can I profile Python code line-by-line?

I believe that's what Robert Kern's line_profiler is intended for. From the link:

File: pystone.py
Function: Proc2 at line 149
Total time: 0.606656 s

Line #      Hits         Time  Per Hit   % Time  Line Contents
==============================================================
   149                                           @profile
   150                                           def Proc2(IntParIO):
   151     50000        82003      1.6     13.5      IntLoc = IntParIO + 10
   152     50000        63162      1.3     10.4      while 1:
   153     50000        69065      1.4     11.4          if Char1Glob == 'A':
   154     50000        66354      1.3     10.9              IntLoc = IntLoc - 1
   155     50000        67263      1.3     11.1              IntParIO = IntLoc - IntGlob
   156     50000        65494      1.3     10.8              EnumLoc = Ident1
   157     50000        68001      1.4     11.2          if EnumLoc == Ident1:
   158     50000        63739      1.3     10.5              break
   159     50000        61575      1.2     10.1      return IntParIO

You could also use pprofile(pypi). If you want to profile the entire execution, it does not require source code modification. You can also profile a subset of a larger program in two ways:

  • toggle profiling when reaching a specific point in the code, such as:

    import pprofile
    profiler = pprofile.Profile()
    with profiler:
        some_code
    # Process profile content: generate a cachegrind file and send it to user.
    
    # You can also write the result to the console:
    profiler.print_stats()
    
    # Or to a file:
    profiler.dump_stats("/tmp/profiler_stats.txt")
    
  • toggle profiling asynchronously from call stack (requires a way to trigger this code in considered application, for example a signal handler or an available worker thread) by using statistical profiling:

    import pprofile
    profiler = pprofile.StatisticalProfile()
    statistical_profiler_thread = pprofile.StatisticalThread(
        profiler=profiler,
    )
    with statistical_profiler_thread:
        sleep(n)
    # Likewise, process profile content
    

Code annotation output format is much like line profiler:

$ pprofile --threads 0 demo/threads.py
Command line: ['demo/threads.py']
Total duration: 1.00573s
File: demo/threads.py
File duration: 1.00168s (99.60%)
Line #|      Hits|         Time| Time per hit|      %|Source code
------+----------+-------------+-------------+-------+-----------
     1|         2|  3.21865e-05|  1.60933e-05|  0.00%|import threading
     2|         1|  5.96046e-06|  5.96046e-06|  0.00%|import time
     3|         0|            0|            0|  0.00%|
     4|         2|   1.5974e-05|  7.98702e-06|  0.00%|def func():
     5|         1|      1.00111|      1.00111| 99.54%|  time.sleep(1)
     6|         0|            0|            0|  0.00%|
     7|         2|  2.00272e-05|  1.00136e-05|  0.00%|def func2():
     8|         1|  1.69277e-05|  1.69277e-05|  0.00%|  pass
     9|         0|            0|            0|  0.00%|
    10|         1|  1.81198e-05|  1.81198e-05|  0.00%|t1 = threading.Thread(target=func)
(call)|         1|  0.000610828|  0.000610828|  0.06%|# /usr/lib/python2.7/threading.py:436 __init__
    11|         1|  1.52588e-05|  1.52588e-05|  0.00%|t2 = threading.Thread(target=func)
(call)|         1|  0.000438929|  0.000438929|  0.04%|# /usr/lib/python2.7/threading.py:436 __init__
    12|         1|  4.79221e-05|  4.79221e-05|  0.00%|t1.start()
(call)|         1|  0.000843048|  0.000843048|  0.08%|# /usr/lib/python2.7/threading.py:485 start
    13|         1|  6.48499e-05|  6.48499e-05|  0.01%|t2.start()
(call)|         1|   0.00115609|   0.00115609|  0.11%|# /usr/lib/python2.7/threading.py:485 start
    14|         1|  0.000205994|  0.000205994|  0.02%|(func(), func2())
(call)|         1|      1.00112|      1.00112| 99.54%|# demo/threads.py:4 func
(call)|         1|  3.09944e-05|  3.09944e-05|  0.00%|# demo/threads.py:7 func2
    15|         1|  7.62939e-05|  7.62939e-05|  0.01%|t1.join()
(call)|         1|  0.000423908|  0.000423908|  0.04%|# /usr/lib/python2.7/threading.py:653 join
    16|         1|  5.26905e-05|  5.26905e-05|  0.01%|t2.join()
(call)|         1|  0.000320196|  0.000320196|  0.03%|# /usr/lib/python2.7/threading.py:653 join

Note that because pprofile does not rely on code modification it can profile top-level module statements, allowing to profile program startup time (how long it takes to import modules, initialise globals, ...).

It can generate cachegrind-formatted output, so you can use kcachegrind to browse large results easily.

Disclosure: I am pprofile author.