Create a Time Series from a CSV file

You can use the zoo package. The code below was writen to be reproducible but in actual practice text="Lines" would be replaced with file="fileName". Also as shown in the question the Date field is ambiguous and you may need to adjust the percent codes if its not day/month/year.

library(zoo)

Lines <- "Date,Time,Value
04/01/2010,07:10,17159
04/01/2010,07:20,4877
04/01/2010,07:30,6078
04/01/2010,07:40,3105
"

z <- read.zoo(text = Lines, sep = ",", header = TRUE, 
       index = 1:2, tz = "", format = "%d/%m/%Y %H:%M")

which gives:

> z
2010-01-04 07:10:00 2010-01-04 07:20:00 2010-01-04 07:30:00 2010-01-04 07:40:00 
              17159                4877                6078                3105