Can I play Dota 2 on Linux?

Dota 2 is now supported on Linux!

Not only can you now watch games in client on Mac and Linux computers, the complete Dota 2 game is available for Mac and Linux players. Grab it here.

Yes, actually. Wine will run Steam and DotA 2 (along with other source games, including TF2) fine. There are some particle rendering and lighting issues though. Also, performance varies by desktop environment. XFCE and LXDE have the best track record, where Unity and KDE have the worse. There is also a pulseaudio bug that crashes your game when it tries to get direct access to sound hardware, there is a fix. More at the wine appdb entry.